DNA

Part:BBa_K4585004:Design

Designed by: Siyao Ma   Group: iGEM23_CSU-CHINA   (2023-09-22)


9XUAS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 158
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 158
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 158
    Illegal XhoI site found at 38
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 158
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 158
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Because we have repeated UAS nine times, we should pay attention to avoiding duplicate fragments when designing primer. If you can't avoid duplicate clips, please note the times that UAS in PCR products has been repeated .


Source

UAS is a piece of small DNA sequence that can be recognized and combined by GAL4. In our design,we have made some alterations. Firstly, we have repeated the UAS before promoter for 9 times. The purpose of constructing 9XUAS-Ad is to enhance the recognition and combination of GAL4, improve the efficiency of downstream target gene activation. Secondly, we have used Ad promoter as promoter to make it able to function in eukaryotic cells and improve the expression efficiency after the activation of VP64.

References